Publications

Highlights

(For a full list see below or go to Chus Perteguer ORCID or to Javier Sotillo Google Scholar, or ORCID)

 

2021

Multiple-bead assay for the differential serodiagnosis of neglected human cestodiases: Neurocysticercosis and cystic echinococcosis
Hernández-González A, González-Bertolín B, Urrea L, Fleury A, Ferrer E, Siles-Lucas M, Tamarozzi F and Perteguer MJ.
PLOS Neglected Tropical Diseases 16(1):e0010109

Immunomics-guided discovery of serum and urine antibodies for diagnosing urogenital schistosomiasis: a biomarker identification study
Mark S Pearson, Bemnet A Tedla, Gebeyaw G Mekonnen, Carla Proietti, Luke Becker, Rie Nakajima, Al Jasinskas, Denise L Doolan, Abena S Amoah, Stefanie Knopp, David Rollinson, Said M Ali, Fatma Kabole, Cornelis H Hokke, Akim A Adegnika, Matt A Field, Govert van Dam, Paul L A M Corstjens, Takafira Mduluza, Francisca Mutapi, Claude Oeuvray, Beatrice Greco, Sujittra Chaiyadet, Thewarach Laha, Pengfei Cai, Donald P McManus, Maria Elena Bottazzi, Philip L Felgner, Javier Sotillo, Alex Loukas
The Lancet Microbe Volume 2, ISSUE 11, e617-e626, November 01, 2021

Insights into the functional expansion of the astacin peptidase family in parasitic helminths
Martin-Galiano Antonio J, Sotillo, J.
International Journal for Parasitology, October 2021.

Immunomics-Guided Antigen Discovery for Praziquantel-Induced Vaccination in Urogenital Human Schistosomiasis
Pearson Mark S., Tedla Bemnet A., Becker Luke, Nakajima Rie, Jasinskas Al, Mduluza Takafira, Mutapi Francisca, Oeuvray Claude, Greco Beatrice, Sotillo Javier, Felgner Philip L., Loukas Alex
Frontiers in Immunology 12:663401, 2021

Rodent Models for the Study of Soil-Transmitted Helminths: A Proteomics Approach
Karen Montano, Carmen Cuellar, y Javier Sotillo
Frontiers in Cellular and Infection Microbiology 11:639573, 2021

Proteomic Approaches to Drive Advances in Helminth Extracellular Vesicle Research
Karen Montano, Alex Loukas, y Javier Sotillo
Molecular Immunology 131(marzo):1-5, 2021

Gut-Microbiota-Derived Extracellular Vesicles: Overlooked Mediators in Host-Helminth Interactions?
Cortes, Alba, Javier Sotillo, Gabriel Rinaldi, y Cinzia Cantacessi
Trends in Parasitology 37(8):690-93, 2021

Proteomic Identification of the Contents of Small Extracellular Vesicles from in Vivo Plasmodium yoelii Infection
De Sousa, Karina P., Jeremy Potriquet, Jason Mulvenna, Javier Sotillo, Penny L. Groves, Alex Loukas, Simon H. Apte, y Denise L. Doolan
International Journal for Parasitology, July 2021

First Evidence of Antibodies Against Lloviu Virus in Schreiber’s Bent-Winged Insectivorous Bats Demonstrate a Wide Circulation of the Virus in Spain
Ramírez de Arellano E, Sanchez-Lockhart M, Perteguer MJ, Bartlett M, Ortiz M, Campioli P, Hernández A, Gonzalez J, Garcia K, Ramos M, Jiménez-Clavero MÁ, Tenorio A, Sánchez-Seco MP, González F, Echevarría JE, Palacios G, Negredo A.
Viruses. 2019 Apr 19;11(4):360

Hookworms Evade Host Immunity by Secreting a Deoxyribonuclease to Degrade Neutrophil Extracellular Traps
Bouchery T, Moyat M, Sotillo J, Silverstein S, Volpe B, Coakley G, Tsourouktsoglou TD, Becker L, Shah K, Kulagin M, Guiet R, Camberis M, Schmidt A, Seitz A, Giacomin P, Le Gros G, Papayannopoulos V, Loukas A, Harris NL.
Cell Host Microbe. 2020 Feb 12;27(2):277-289.e6.

2020

The Protein and MicroRNA Cargo of Extracellular Vesicles from Parasitic Helminths - Current Status and Research Priorities
Sotillo, Javier, Mark W. Robinson, Michael J. Kimber, Marcela Cucher, Maria Eugenia Ancarola, Peter Nejsum, Antonio Marcilla, Ramon M. Eichenberger, y Lucienne Tritten
International Journal for Parasitology 50(9):635-45, 2020

Gastrointestinal Helminth Infection Improves Insulin Sensitivity, Decreases Systemic Inflammation, and Alters the Composition of Gut Microbiota in Distinct Mouse Models of Type 2 Diabetes
Khudhair, Zainab, Rafid Alhallaf, Ramon M. Eichenberger, Jen Whan, Andreas Kupz, Matt Field, Lutz Krause, David Wilson, Norelle Daly, Paul Giacomin, Javier Sotillo and Alex Loukas
Frontiers in Endocrinology 11:606530, 2020

Schistosoma haematobium Extracellular Vesicle Proteins Confer Protection in a Heterologous Model of Schistosomiasis
Mekonnen, Gebeyaw G., Bemnet A. Tedla, Darren Pickering, Luke Becker, Lei Wang, Bin Zhan, Maria Elena Bottazzi, Alex Loukas, Javier Sotillo, y Mark S. Pearson
Vaccines 8 (3), 2020

Evaluation of the sensitivity and specificity of GST-tagged recombinant antigens 2B2t, Ag5t and DIPOL in ELISA for the diagnosis and follow up of patients with cystic echinococcosis
Carlos Sanchez-Ovejero, Eylem Akdur, R. Manzano-Román, Ana Hernández González, González-Sánchez, David Becerro-Recio, Javier González-Miguel, Okan Akhan, Carmen M. Cretu, Kamenna Vutova, Francesca Tamarozzi, Mara Mariconti, Enrico Brunetti, Ambra Vola, Massimo Fabiani, Adriano Casulli, Mar Siles-Lucas
PLoS Neglected Tropical Diseases November 30, 2020

Liver fluke granulin promotes extracellular vesicle-mediated crosstalk and cellular microenvironment conducive to cholangiocarcinoma
Arunsan P, Chaidee A, Cochran CJ, Mann VH, Tanno T, Kumkhaek C, Smout MJ, Karinshak SE, Rodpai R, Sotillo J, Loukas A, Laha T, Brindley PJ, Ittiprasert W.
Neoplasia. 2020 May;22(5):203-216

Hookworms Evade Host Immunity by Secreting a Deoxyribonuclease to Degrade Neutrophil Extracellular Traps
Bouchery T, Moyat M, Sotillo J, Silverstein S, Volpe B, Coakley G, Tsourouktsoglou TD, Becker L, Shah K, Kulagin M, Guiet R, Camberis M, Schmidt A, Seitz A, Giacomin P, Le Gros G, Papayannopoulos V, Loukas A, Harris NL.
Cell Host Microbe. 2020 Feb 12;27(2):277-289.e6.

Proteomic analysis of two populations of Schistosoma mansoni-derived extracellular vesicles: 15k pellet and 120k pellet vesicles
Kifle DW, Pearson MS, Becker L, Pickering D, Loukas A, Sotillo J.
Mol Biochem Parasitol. 2020 Mar;236:111264

Partial protection with a chimeric tetraspanin-leucine aminopeptidase subunit vaccine against Opisthorchis viverrini infection in hamsters.
Thi Phung L, Chaiyadet S, Hongsrichan N, Sotillo J, Dinh Thi Dieu H, Quang Tran C, Brindley PJ, Loukas A, Laha T.
Acta Trop. 2020 Apr;204:105355.

Set up of an in vitro model to study early host-parasite interactions between newly excysted juveniles of Fasciola hepatica and host intestinal cells using a quantitative proteomics approach.
González-Miguel J, Becerro-Recio D, Sotillo J, Simón F, Siles-Lucas M.
Vet Parasitol. 2020 Feb;278:109028.

Older publications

First Evidence of Antibodies Against Lloviu Virus in Schreiber’s Bent-Winged Insectivorous Bats Demonstrate a Wide Circulation of the Virus in Spain
Ramírez de Arellano E, Sanchez-Lockhart M, Perteguer MJ, Bartlett M, Ortiz M, Campioli P, Hernández A, Gonzalez J, Garcia K, Ramos M, Jiménez-Clavero MÁ, Tenorio A, Sánchez-Seco MP, González F, Echevarría JE, Palacios G, Negredo A.
Viruses. 2019 Apr 19;11(4):360

In-plate recapturing of a dual-tagged recombinant Fasciola antigen (FhLAP) by a monoclonal antibody (US9) prevents non-specific binding in ELISA.
Orbegozo-Medina RA, Martínez-Sernández V, Perteguer MJ, Hernández-González A, Mezo M, González-Warleta M, Romarís F, Paniagua E, Gárate T, Ubeira FM.
PLoS One. 2019 Feb 1;14(2):e0211035

Antibody responses to chimeric peptides derived from parasite antigens in mice and other animal species
Orbegozo-Medina RA, Martínez-Sernández V, Folgueira I, Mezo M, González-Warleta M, Perteguer MJ, Romarís F, Leiro JM, Ubeira FM.
Mol Immunol. 2019 Feb;106:1-11

Epidemiological Scenario of Anisakidosis in Spain Based on Associated Hospitalizations: The Tip of the Iceberg
Herrador Z, Daschner Á, Perteguer MJ, Benito A.
Clin Infect Dis. 2019 Jun 18;69(1):69-76

Interruption of onchocerciasis transmission in Bioko Island: Accelerating the movement from control to elimination in Equatorial Guinea
Herrador Z, Garcia B, Ncogo P, Perteguer MJ, Rubio JM, Rivas E, Cimas M, Ordoñez G, de Pablos S, Hernández-González A, Nguema R, Moya L, Romay-Barja M, Garate T, Barbre K, Benito A.
PLoS Negl Trop Dis. 2018 May 3;12(5):e0006471

Comparison of recombinant cathepsins L1, L2, and L5 as ELISA targets for serodiagnosis of bovine and ovine fascioliasis.
Martínez-Sernández V, Perteguer MJ, Hernández-González A, Mezo M, González-Warleta M, Orbegozo-Medina RA, Romarís F, Paniagua E, Gárate T, Ubeira FM.
Parasitol Res. 2018 May;117(5):1521-1534.

HDP2: a ribosomal DNA (NTS-ETS) sequence as a target for species-specific molecular diagnosis of intestinal taeniasis in humans
Flores MD, Gonzalez LM, Hurtado C, Motta YM, Domínguez-Hidalgo C, Merino FJ, Perteguer MJ, Gárate T.
Parasit Vectors. 2018 Feb 27;11(1):117

Novel cholinesterase paralogs of Schistosoma mansoni have perceived roles in cholinergic signalling and drug detoxification and are essential for parasite survival.
Tedla BA, Sotillo J, Pickering D, Eichenberger RM, Ryan S, Becker L, Loukas A, Pearson MS.
PLoS Pathog. 2019 Dec 6;15(12):e1008213.

Recombinant Opisthorchis viverrini tetraspanin expressed in Pichia pastoris as a potential vaccine candidate for opisthorchiasis.
Phung LT, Chaiyadet S, Hongsrichan N, Sotillo J, Dieu HDT, Tran CQ, Brindley PJ, Loukas A, Laha T.
Parasitol Res. 2019 Dec;118(12):3419-3427

Recent advances on the immunobiology of Bithynia spp. hosts of Opisthorchis viverrini
Suwannatrai K, Suwannatrai AT, Loukas A, Sotillo J.
Dev Comp Immunol. 2020 Jan;102:103460.

Trematode Genomics and Proteomics.
Sotillo J, Pearson MS, Loukas A.
Adv Exp Med Biol. 2019;1154:411-436.

Vaccination of hamsters with Opisthorchis viverrini extracellular vesicles and vesicle-derived recombinant tetraspanins induces antibodies that block vesicle uptake by cholangiocytes and reduce parasite burden after challenge infection.
haiyadet S, Sotillo J, Krueajampa W, Thongsen S, Brindley PJ, Sripa B, Loukas A, Laha T.
PLoS Negl Trop Dis. 2019 May 28;13(5):e0007450.

In-depth proteomic characterization of Schistosoma haematobium: Towards the development of new tools for elimination
Sotillo J, Pearson MS, Becker L, Mekonnen GG, Amoah AS, van Dam G, Corstjens PLAM, Murray J, Mduluza T, Mutapi F, Loukas A.
PLoS Negl Trop Dis. 2019 May 15;13(5):e0007362.

Programmed knockout mutation of liver fluke granulin attenuates virulence of infection-induced hepatobiliary morbidity
Arunsan P, Ittiprasert W, Smout MJ, Cochran CJ, Mann VH, Chaiyadet S, Karinshak SE, Sripa B, Young ND, Sotillo J, Loukas A, Brindley PJ, Laha T.
Elife. 2019 Jan 15;8:e41463.

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
Théry C, Witwer KW, Aikawa E, et al.
J Extracell Vesicles. 2018 Nov 23;7(1):1535750.

Opisthorchis viverrini Proteome and Host-Parasite Interactions.
Suttiprapa S, Sotillo J, Smout M, Suyapoh W, Chaiyadet S, Tripathi T, Laha T, Loukas A.
Adv Parasitol. 2018;102:45-72.

Structural Variants of a Liver Fluke Derived Granulin Peptide Potently Stimulate Wound Healing
Dastpeyman M, Bansal PS, Wilson D, Sotillo J, Brindley PJ, Loukas A, Smout MJ, Daly NL.
J Med Chem. 2018 Oct 11;61(19):8746-8753.

Immunobiology of parasitic worm extracellular vesicles
Eichenberger RM, Sotillo J, Loukas A.
Immunol Cell Biol. 2018 May 29.

Hookworm Secreted Extracellular Vesicles Interact With Host Cells and Prevent Inducible Colitis in Mice.
Eichenberger RM, Ryan S, Jones L, Buitrago G, Polster R, Montes de Oca M, Zuvelek J, Giacomin PR, Dent LA, Engwerda C, Field M, Sotillo J, Loukas A.
Front Immunol. 2018 Apr 30;9:850

The NLRP3 Inflammasome Suppresses Protective Immunity to Gastrointestinal Helminth Infection.
Alhallaf R, Agha Z, Miller CM, Robertson AAB, Sotillo J, Croese J, Cooper MA, Masters SL, Kupz A, Smith NC, Loukas A, Giacomin PR.
Cell Rep. 2018 Apr 24;23(4):1085-1098

Adaptation of the secretome of Echinostoma caproni may contribute to parasite survival in a Th1 milieu.
Cortés A, Muñoz-Antolí C, Álvarez-Izquierdo M, Sotillo J, Esteban JG, Toledo R.
Parasitol Res. 2018 Apr;117(4):947-957.

Characterization of Trichuris muris secreted proteins and extracellular vesicles provides new insights into host-parasite communication.
Eichenberger RM, Talukder MH, Field MA, Wangchuk P, Giacomin P, Loukas A, Sotillo J.
J Extracell Vesicles. 2018 Jan 21;7(1):1428004.

Extracellular vesicles from parasitic helminths and their potential utility as vaccines
Mekonnen GG, Pearson M, Loukas A, Sotillo J.
Expert Rev Vaccines. 2018 Mar;17(3):197-205.

Fasciola spp: Mapping of the MF6 epitope and antigenic analysis of the MF6p/HDM family of heme-binding proteins
Martínez-Sernández V, Perteguer MJ, Mezo M, González-Warleta M, Gárate T, Valero MA, Ubeira FM.
PLoS One. 2017 Nov 21;12(11):e0188520.

Recombinant vs native Anisakis haemoglobin (Ani s 13): Its appraisal as a new gold standard for the diagnosis of allergy
González-Fernández J, Rivas L, Luque-Ortega JR, Núñez-Ramírez R, Campioli P, Gárate T, Perteguer MJ, Daschner A, Cuéllar C.
Exp Parasitol. 2017 Oct;181:119-129.

Present status of laboratory diagnosis of human taeniosis/cysticercosis in Europe.
Gómez-Morales MA, Gárate T, Blocher J, Devleesschauwer B, Smit GSA, Schmidt V, Perteguer MJ, Ludovisi A, Pozio E, Dorny P, Gabriël S, Winkler AS.
Eur J Clin Microbiol Infect Dis. 2017 Nov;36(11):2029-2040.

Comparison of T24H-his, GST-T24H and GST-Ts8B2 recombinant antigens in western blot, ELISA and multiplex bead-based assay for diagnosis of neurocysticercosis.
Hernández-González A, Noh J, Perteguer MJ, Gárate T, Handali S.
Parasit Vectors. 2017 May 15;10(1):237.

Delineating distinct heme-scavenging and -binding functions of domains in MF6p/helminth defense molecule (HDM) proteins from parasitic flatworms.
Martínez-Sernández V, Mezo M, González-Warleta M, Perteguer MJ, Gárate T, Romarís F, Ubeira FM.
J Biol Chem. 2017 May 26;292(21):8667-8682.

Evaluation of onchocerciasis seroprevalence in Bioko Island (Equatorial Guinea) after years of disease control programmes.
Hernández-González A, Moya L, Perteguer MJ, Herrador Z, Nguema R, Nguema J, Aparicio P, Benito A, Gárate T.
Parasit Vectors. 2016 Sep 20;9(1):509

Evidence for Suppression of Onchocerciasis Transmission in Bioko Island, Equatorial Guinea
Moya L, Herrador Z, Ta-Tang TH, Rubio JM, Perteguer MJ, Hernandez-González A, García B, Nguema R, Nguema J, Ncogo P, Garate T, Benito A, Sima A, Aparicio P.
PLoS Negl Trop Dis. 2016 Jul 22;10(7):e0004829.

ANISERP: a new serpin from the parasite Anisakis simplex
Valdivieso E, Perteguer MJ, Hurtado C, Campioli P, Rodríguez E, Saborido A, Martínez-Sernández V, Gómez-Puertas P, Ubeira FM, Gárate T.
Parasit Vectors. 2015 Jul 28;8:399.

The MF6p/FhHDM-1 major antigen secreted by the trematode parasite Fasciola hepatica is a heme-binding protein.
Martínez-Sernández V, Mezo M, González-Warleta M, Perteguer MJ, Muiño L, Guitián E, Gárate T, Ubeira FM.
J Biol Chem. 2014 Jan 17;289(3):1441-56

Analysis of an expressed sequence tag library from Dicrocoelium dentriticum
Martínez-Ibeas AM, Perteguer MJ, González-Lanza C, Gárate T, Manga-González MY.
Exp Parasitol. 2013 Oct;135(2):287-96.

Ddi1-like protein from Leishmania major is an active aspartyl proteinase.
Perteguer MJ, Gómez-Puertas P, Cañavate C, Dagger F, Gárate T, Valdivieso E.
Cell Stress Chaperones. 2013 Mar;18(2):171-81

Assessing the validity of an ELISA test for the serological diagnosis of human fascioliasis in different epidemiological situations
Valero MA, Periago MV, Pérez-Crespo I, Rodríguez E, Perteguer MJ, Gárate T, González-Barberá EM, Mas-Coma S
Trop Med Int Health. 2012 May;17(5):630-6.

Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis
Martínez-Sernández V, Muiño L, Perteguer MJ, Gárate T, Mezo M, González-Warleta M, Muro A, Correia da Costa JM, Romarís F, Ubeira FM.
PLoS Negl Trop Dis. 2011 Nov;5(11):e1376